Kisspeptin-8 Induces Anxiety-Like Behavior and Hypolocomotion by Activating the HPA Axis and Increasing GABA Release in the Nucleus Accumbens in Rats

Kisspeptins (Kp) are RF-amide neuropeptide regulators of the reproductive axis that also influence anxiety, locomotion, and metabolism. We aimed to investigate effects intracerebroventricular Kp-8 (an N-terminally truncated octapeptide) treatment in Wistar rats. Elevated plus maze (EPM), computerized open field (OF), marble burying (MB) tests were performed for assessment behavior. Serum LH corticosterone levels determined assess kisspeptin1 receptor (Kiss1r) activation hypothalamic-pituitary-adrenal (HPA) stimulation, respectively. GABA release from nucleus accumbens (NAc) dopamine ventral tegmental area (VTA) NAc measured via ex vivo superfusion. decreased arm time entries EPM, raised concentration, pointing an anxiogenic effect. Moreover, decrease delayed increase immobility accompanied by reduced ambulatory activity OF, reduction interactions with marbles show suppressed exploratory spontaneous locomotion. The might be background hypolocomotion inhibiting VTA-NAc dopaminergic circuitry. As it could activate Kiss1r stimulate axis. is associated hyperlocomotion, more likely FF involved suppression locomotor activity.